PCR-SSCP检测大肠癌组织中p53、K-ras基因的突变

Study of p53 and K-ras Gene Mutation in Colorectal Carcinoma by PCR-SSCP

目的 探讨p5 3、K -ras基因突变与大肠癌发生、发展的关系。方法 应用PCR -SSCP方法检测 68例大肠癌和癌旁组织以及 2 0例正常组织中 p5 3、K -ras基因突变情况。 结果 大肠癌组织中 p5 3、K -ras基因突变率分别为 47.1% (3 2 68)和44 .1% (3 0 68) ,明显高于癌旁组织 (13 .2 % ,9 68;7.4% ,5 68) ,2 0例正常组织中未检出 p5 3、K -ras基因突变。伴有淋巴结及远处转移的大肠癌者 ,其 p5 3、K -ras基因突变率明显高于无淋巴结及远处转移者 ;p5 3、K -ras基因突变与大肠癌组织学类型无关。结论 p5 3、K -ras基因突变与大肠癌发生、发展有密切关系 ,其在细胞癌变中起重要作用 ,可作为评估大肠癌转移的分子生物学指标之一。

Objective To study the relation between p53,K ras gene mutation to the occurrence and development of colorectal carcinoma.Methods The positive rates of p53 and K ras gene mutation in 68 cases of cancer tissue,peritumoral tissue samples and 20 normal controls were studied by PCR SSCP.Results The positive rates of p53 and K ras gene mutation in cancer tissue (47.1% ,32/68;44.1%,30/68) were significantly higher than in peritumoral tissue(13.2%,9/68;7.4%,5/68).p53,K ras gene mutation were not detectable in 20 normal tissues.The positive rate of p53 and K ras gene mutation in the group with lymph nodes and distant metastasis was markedly higher than those in the group without metastasis,but their relation to the histologic type was not significant.Conclusion A close correlation can be present between p53,K ras gene mutation and the occurrence and development of colorectal carcinoma.p53 and K ras gene mutation in cancer tissue may play an important role during colorectal carcinogenesis,and could be regarded as biomolecular index for the evaluation of metastasis in colorectal carcinoma.