烫伤和应用抗生素与肠道白色念珠菌移位的实验研究

Systemic Dissemination of Candida Albicans from Gut after Thermal Injury and Antibiotic Therapy in Rats

本实验共用大鼠160只,动物烫伤后,腹腔注射苯唑青霉素与庆大霉素,管饲标记的白色念珠菌。结果发现:给菌后1小时,内脏标记菌的总检出率为30%,6小时达70%;其中肝和肠系膜淋巴结检出率最高分别为64%和80%;尿液直接镜检阳性率为40%,较血,尿培养敏感。烫伤和应用抗生素对肠道念珠菌的移位有协同作用。

A total of one hundred and sixty rats were fed with isothiocyanatelabelled candida albicans and then randomly divided into four groups: simple burn (30% TBSA full-thickness scald burn was created on the back), simple antibiotic therapy (oxacillin combind with gentamicin were injected intraperitineally), 30% full-thickness burn plus antibiotic therapy and the control group. The animals were sacrificed and examined at different time phases. The labelled candida albicans were traced in the mesentery lymph nodes, liver, spleen, kidney, lung, blood stream and urine. The labelled candida albicans could be demontrated from 30% of visceral organs at one hour and from 70% at six hours after burn plus antibiotic therapy, while the mesentery lymph node and liver were the primary organs invaded. The process of candida translocation through the intestinal wall was displayed as adhesion and then invasion. For comprative study of the methods or the early diagnosis of systemic dissemination of candida albicans, blood cultures, urine cultures and direct examination of urine under microscope were performed. The detectable rate was only 8% in blood culture and 21.8% in urine culture, however, it was 40% in direct examination of urine, the later method is therefore much more sensitive than the other two.