微生物去毒剂去除饲料中真菌毒素的猪体试验

Effects of a Microbiological Detoxification Agent on Mycotoxin in Feed Pigs

研究用生物学方法取代理化方法去除饲料中污染的霉菌毒素。采用德国某公司提供的新型微生物去毒剂BH ,加入到含有脱氧雪腐镰菌醇 (deoxynivalenol,DON)的霉变小麦中 ,用仔猪和生长猪分别作动物试验测定其去毒效果。结果显示 ,DON显著降低仔猪和生长猪的增重速度 ,对生长猪增重的影响大于对仔猪的影响 ;添加BH则可显著减弱DON对仔猪和生长猪增重的影响 ,证明在动物体内BH可减弱DON的毒性作用 ;单独添加BH组猪的增重与对照组无差异 ,表明BH对动物增重无不利影响。DON对仔猪某些血液生化指标有一定影响 ,尤以谷 草转氨酶和谷 丙转氨酶的升高最为显著。体外试验显示 ,BH在液体培养基中 ,可降低DON含量。霉变饲料中添加BH ,未经动物胃肠道作用 ,其内的DON含量与添加BH之前相比 。

Biological method is an ideal way to remove the toxin from contaminated feed compared with both physical and chemical methods. A new microbiological agent, BH was used to decrease the toxicity of mildewed wheat containing Deoxynivalenol (DON) at 22.7 mg per kg feed. Experimental animals included two batches of 24 pigs (5 kg) and 48 pigs (30 kg). Each batch was randomly divided into 4 groups fed with routine diets supplemented with different agents as follows: Group 1, +DON; Group 2, +DON+BH; Group 3, +BH; Group 4, just the routine diet as control. The weight gain was detected every 2 weeks, while blood biochemical items every 3 weeks during the experimental period of 6 weeks. The results showed that DON significantly reduced the weight gain of both batches. BH greatly alleviated the harm of DON to the animals. However, BH alone has no influence on weight gain. Furthermore, DON increased significantly some biochemical items, such as AST and GPT. In vitro, BH reduced the amount of DON in liquid medium, while BH added mildew feed did not apparently affect the content of DON in feed if they did not pass the gastrointestine.