铁破锣皂苷O和P的结构及其药理活性

STRUCTURES AND PHARMACOLOGICAL ACTIVITIES OF BEESIOSIDES O AND P

目的 研究我国特有药用植物铁破锣 [Bessiacalthaefolia (Maxim .)Ulhr.]根茎的化学成分。方法 利用各种色谱技术进行分离 ,根据化合物的光谱数据 (IR ,MS ,1 HNMR ,1 3CNMR ,2DNMR)和化学方法鉴定其结构 ,并对所得单体成分进行药理活性筛选。结果 从甘肃产铁破锣根茎的氯仿萃取物中分离得到 2个化合物 ,分别鉴定为 :(2 0S ,2 4S) 15α acetoxy 16 β ,2 4 ;2 0 ,2 4 diepoxy 9,19 cyclolanostane 3β ,2 5 diol 3 O β D xylopyranoside (I)和 (2 0S ,2 4R) 15α acetoxy 9,19 cyclolanostane 3β ,16 β ,2 0 ,2 4 ,2 5 pentaol 3 O β D xylopyranoside (II) ,分别命名为铁破锣皂苷O(beesiosideO)和铁破锣皂苷P(beesiosideP)。结论 I和II为新化合物 ,I有免疫抑制、抑制血管生成和抑制成骨细胞增殖活性。

AIM To investigate the chemical constituents of the rhizomes of Beesia calthaefolia native to China in order to obtain a more comprehensive understanding of its effective components. METHODS Compounds were isolated by column chromatography with silica gel. Their structures were elucidated by spectral analysis and chemical evidence. Compounds identified were subjected to pharmacological evaluation. RESULTS Two novel compounds were isolated and identified as (20 S ,24 S ) 15α acetoxy 16β,24;20,24 diepoxy 9,19 cyclolanostane 3β,25 diol 3 O β D xylopyranoside (I) and (20 S ,24R) 15α acetoxy 9,19 cyclolanostane 3β,16β,20,24,25 pentaol 3 O β D xylopyranoside (II), named beesioside O and beesioside P. CONCLUSION Compounds I and II are new compounds. Compounds I exhibited immunosuppressive activity and could inhibit angiogenesis as well as inhibit the proliferation of osteoblast. Compound II displayed remarkable inhibition activity against calcium channel receptor.