环糊精修饰混合胶束电动色谱法测定大黄中6种有效成分

DETERMINATION OF SIX EFFECTIVE COMPONENTS IN RHEUM BY CYCLODEXTRIN MODIFIED MICELLAR ELECTROKINETIC CHROMATOGRAPHY

目的 分离、测定大黄样品中 6种有效成分。方法 用 2 0mmol·L- 1 硼砂缓冲溶液 (含 2 0mmol·L- 1 脱氧胆酸钠、2 0mmol·L- 1 牛磺胆酸钠和 15mmol·L- 1 β 环糊精 ) ,通过环糊精修饰的混合胶束电动色谱内标定量法测定。 结果 在 2 5min内分离出芦荟大黄素、大黄酸、大黄素、土大黄苷、大黄素甲醚和大黄酚 ,对缓冲溶液pH、脱氧胆酸钠、牛磺胆酸钠和 β 环糊精浓度等分离条件进行了优化。考察了方法的线性行为、精密度和回收率 ,并对生大黄和蒙古大黄样品进行了测定。结论 本法可作为鉴别大黄优劣的有效方法之一。

AIM To determine six effective components (aloe emodin, rhein, emodin, rhaponticin, physcion and chrysophanol) in Rheum . METHODS Using buffer solution containing 20 mmol·L -1 borax, 20 mmol·L -1 sodium deoxycholate (SDC), 20 mmol·L -1 sodium taurocholate (STC), 15 mmol·L -1 β cyclodextrin (β CD) and O phthalic acid as the internal standard, the six components were determined by cyclodextrin modified micellar electrokinetic chromatography. RESULTS In less than 25 min, aloe emodin, rhein, emodin, rhaponticin, physcion and chrysophanol were separated. The separation conditions were optimized by adjusting buffer pH, concentrations of SDC, STC and β CD. The linearity ranges of aloe emodin, rhein, emodin, rhaponticin, physcion and chrysophanol were 4～34, 5～40, 4～60, 5～80, 6～90 and 5～85 μg·mL -1 respectively. Relative standard deviation (RSD) of the method was less than 2 2%. The recoveries of aloe emodin, rhein, emodin, rhaponticin, physcion and chrysophanol were 100 0%, 98 3%, 100 4%, 94 6% 95 2% and 93 8% respectively. Raw Rheum , Mongolian Rheum and Rheum tanguticum samples were analyzed. CONCLUSION This method can be an effective one for identification of Rhubarb.