TT病毒粪-口传播途径的初步探讨

A PRELIMINARY STUDY ON FECAL-ORAL TRANSMISSION OF TT VIRUS

①目的 探讨TT病毒 (TTV)经粪 口传播的可能性。②方法 采用半巢式聚合酶链反应 ,检测 6例TTV感染的肝病病人粪便标本中TTV DNA ,测序 ,与N2 2和TA2 78株核苷酸比较并进行基因分型 ,探讨粪便中TTV DNA检出率与血清中病毒滴度的关系。③结果 血清病毒滴度较高的 2例病人 (1 0 7/L ,1 0 6 /L)的粪便标本中检出了TTV DNA ,滴度分别为 1 0 7/L ,1 0 5/L ,基因型分别为G1a,G1b;在血清病毒滴度为 1 0 4 /L的 4例病人中 ,仅1例在粪便中检出了TTV DNA ,滴度为 1 0 4 /L ,基因型为G2 .④结论 肝病病人粪便标本中TTV含量与血清滴度直接相关 ,TTV具有经粪

Objective\ To study the possibility of feca l oral transmission of TT virus(TTV). \ Methods\ Paired feces and serum samples from 6 patients with type B and/or type C hepatitis were tested for TTV DNA and their titers by PCR with s eminested primers. Genotypes were determined by their sequences compared with th e original N22 and TA278 strains. \ ResultsTTV DNA was detected in fecal specimen of two patients wh o had high viral titers in serum(10 7/L,10 6/L); the genotypes were G 1a and G 1b , respectively. In 4 patients with viral titer of 10 4/L, TTV DNA was detected in only one fecal specimen. The genotype was G 2.\ Conclusion\ The amount of TTV in fecal specimens of patients with hepatitis is directly correlated to serum titer, indicating that TTV can be tran smitted by fecal oral route. [