润肠宁神膏中总黄酮含量测定

Content Determination of Total Flavones in Runchang Ningshen Extract

【目的】系统优化中药复方制剂润肠宁中总黄酮含量的测定方法及测定条件 ,控制润肠宁神膏制剂质量。【方法与结果】以芦丁为对照品 ,用体积分数为 70 %乙醇回流提取 3次 ,得最佳供试品溶液浓度 2 6 2mg/g ;加亚硝酸钠、硝酸铝溶液在碱性条件下显色 ,采用紫外 -分光光度法于 5 11nm波长处测定溶液吸收度 ,得回归方程为 :Y =0 0 11X - 0 0 0 14 ,r =0 9999,在 8～ 4 0 μg范围内呈良好线性关系 ;计算得总黄酮平均回收率为 94 6 3%。【结论】本方法操作简便 ,无干扰 ,重复性好 。

To establish a method for the determination of the content of total flavones and the quality control of Runchang Ningshen Extract. [Methods & Results]With rutin as control,the optimal concentration of Runchang Ningshen Extract was obtained by refluxing for three times with 70% ethanol.Ultraviolet spectrophotometry was used.The regression equation was induced at detecting wavelength being 511nm and with sodium nitrite and aluminium nitrate as color developing agent.There was a good linearity (r=0 9999)in the range of 8 μg.The average recovery of total flavones was 94 63%(RSD=2 58%,n=5).[Conclusion]This method is simple and reproducible and can serve as the standard for controlling the quality of Runchang Ningshen Extract.