人参皂苷Rg3对肺癌诱导血管内皮细胞增殖的抑制作用

Inhibitory effect of panaxoside Rg3 on vascular endothelial cell proliferation induced by lung adenocarcinoma

目的 :探讨人参皂苷Rg3对肺癌诱导血管内皮细胞 (VEC)增殖的抑制作用。 方法 :用MTT法检测不同浓度Rg3对VEC、肺腺癌SPC A 1细胞增殖及SPC A 1细胞诱导VEC增殖的影响。结果 :Rg3浓度为 0 .0 313～0 .5mmol·L- 1 时 ,对VEC及SPC A 1细胞增殖没有直接影响 (P >0 .0 5 ) ;经 0 .0 313～ 0 .5mmol·L- 1 Rg3作用后 ,SPC A 1细胞条件培养液对VEC的增殖作用也不受影响 (P >0 .0 5 ) ;Rg3浓度为 0 .12 5～ 0 .5mmol·L- 1 时 ,其对SPC A 1细胞条件培养液诱导的VEC增殖有抑制作用 (P <0 .0 1) ,抑制率为 16 .4 4 %～ 5 7.5 3%。结论 :人参皂苷Rg3对肺癌细胞条件培养液诱导的VEC增殖具有抑制作用。

Objective:To investigate the inhibitory effect of panaxoside Rg3 on vascular endothelial cell (VEC) proliferation induced by lung adenocarcinoma.Methods:Inhibitory effects of panaxoside Rg3 on proliferation of VEC,lung adenocarcinoma SPC A 1 cell and VEC proliferation induced by SPC A 1 cell were analyzed with MTT method.Results:Panaxoside Rg3 in the range of 0.0313～0.5 mmol·L -1 had no direct influence on VEC and SPC A 1 cell proliferation ( P >0.05),and had also no influence on VEC proliferation induced by SPC A 1 cell conditional cultured medium acted with 0.0313～ 0.5 mmol·L -1 of panaxoside Rg3 ( P >0.05),but panaxoside Rg3 in the range of 0.125～ 0.5 mmol·L -1 inhibited VEC proliferation induced by conditional cultured medium of SPC A 1 cell ( P < 0.01),the inhibitory rate was 16.44%～57.53%.Conclusion:Panaxoside Rg3 can inhibit VEC proliferation induced by conditional cultured medium of lung adenocarcinoma cell.