银环蛇毒素对白血病K562细胞株的毒性作用研究

Research on the toxicological effects of the crue venom of Bungarus multicinctus on K562 tumor cells

目的 探讨银环蛇毒素及其若干组分在体外对白血病K5 6 2细胞株的毒性作用。方法 采用阳离子交换层析法分离纯化银环蛇粗毒 ,应用MTT法、流式细胞术等研究毒素对K5 6 2细胞株的杀伤和凋亡作用。结果 研究发现 ,银环蛇毒素在浓度超过 8× 10 3 ng/ml后开始对K5 6 2细胞 (细胞密度约为 1.1× 10 6个 /ml)有杀伤作用 ,浓度为 8× 10 5ng/ml时已有非常强的杀伤作用。结论 这种杀伤作用具有时间和浓度依赖的关系 ,随着毒素处理时间的延长 ,毒素的K5 6 2细胞杀伤作用也越强 ,但是 ,银环蛇粗毒的这种杀伤作用并非是细胞凋亡作用。

Objective To investigate the toxicological effects of the crude venom of Bungarus multicinctus on K562 tumor cells. Methods The crude venom was separated and purified by cation exchange chromatography. The killing effects and apoptosis of venom on K562 was studied by MTT method and flowcytometry. Results The crude venom in more than 8×103ng/ml concentration begins to kill K562 cells (1.1×106/ml) when 8×105ng/ml crude venom was administered. The effect was very strong in 24 h. Conclusion The crude venom of Bungarus multicinctus can kill K562 tumor cells of human CML in a time and dose dependent manner. However, the killing effect of the vemom is not due to apoptosis.