摘要
以赤子爱胜蚓 (EiseniaFoetidaSarigng)整体为材料 ,采用常规碱性磷酸 (酯 )酶 (AKP)纯化方法 ,即正丁醇抽提 ,硫酸铵分级沉淀 ,DEAE 纤维素柱层析 ,SephadexG 10 0及SephadexG 2 0 0凝胶过滤等 ,分离纯化了蚓体中的一种AKP ,经PAGE鉴定达到电泳纯 ,比活性为 4 0 .6 4U/mg蛋白 ,纯化倍数为 5 7.2 4倍。
Alkaline phosphatase (AKP) was purified from the cell extracts of Eisenia Foetida Sarigng using the routine means: n bulanol extraction, ammonium sulfate fractional precipitation, DEAE cellulose chromatography, Sephadex G 100 and Sephadex G 200 gel filtration. The purified AKP was found to be homogeneous by polyacrylamide gel disc electrophoresis. Its specific activity is 40.64 units per milligram of protein, and purification multiple is 57.24 fold.
出处
《西南交通大学学报》
EI
CSCD
北大核心
2002年第5期597-600,共4页
Journal of Southwest Jiaotong University
