Taqman荧光探针技术检测结核分枝杆菌

TAQMAN PCR FLUORESCENCE PROBE TECHNIQUE IN DETECTING MYCOBACTERIUM TUBERCULOSIS

目的 :比较Taqman荧光探针技术与常规PCR电泳技术检测结核分枝杆菌的灵敏度及特异性 ,评价Taq man荧光探针技术 (Taqman技术 )检测临床标本中结核菌的应用价值。方法 :应用细菌培养法 ,常规PCR电泳技术与Taqman技术检测 30 0份临床结核病患者痰标本及 5 0份非结核呼吸系统疾病患者痰标本。结果 :细菌培养法检测结核阳性率为 112 / 30 0 ;常规PCR电泳技术检测结核菌阳性率为 16 9/ 30 0 ,特异性 96 % ;Taq man荧光探针技术检测结核菌阳性率为 14 1/ 30 0 ,特异性 10 0 %。结论 :Taqman荧光探针技术是一种全新的PCR分子杂交模式 ,集PCR扩增、荧光探针杂交、荧光检测一体化 ,在单一管内完成 ,简便、快速、防污染、敏感性高、特异性强 。

Objective:To compare the sensitivity and specificity of Taqman fluorescence probe technique and routine PCR electrophoresis technique,and evaluate the application value of Taqman technique in detecting clinical mycobacterium tuberculosis.Methods:Three hundred sputum samples of tubercular and 50 sputum samples of nontubercular respiratory system disease were detected by bacillary culture method,roution PCR and Taqman technique.Results:The positive rates of culture method,routine PCR and Taqman technique were 112/130,169/300 and 141/300 respectively.The specificity of routine PCR and Taqman technique were 96% and 100% respectively.Conclusion:Taqman technique is a new PCR molecular hybridization model,it is simple,rapid and contaminateproof,and shows a high specificity annd sensitivity.It is an effective method for diagnosing tuberculosis (TB).