基因芯片技术在放射生物学研究领域的应用

The application of radilbiological study by gene chip technique

机体对电离辐射反应非常复杂,受许多分子调节途径控制,p53就是这样的应激途径之一。已经知道它涉及100多个基因的活动,这些应激基因活动具有某种细胞特异性,DNA芯片技术可以完整地研究整个细胞或器官全部基因变化,可以通过基因分析发现对电离辐射的基因反应差异,从而建立一种新的分子放射生物学方法。应用该技术已经初步发现一些新的辐射反应基因活动,在不同基因背景这些基因活动变化很大,提示生物细胞对环境有害因素反应的复杂性及其在正常组织和肿瘤分化增殖过程中基因调控的重要意义,这种技术也可能成为肿瘤治疗和其他环境毒理研究的检测和预测方法。

The responses to ionizing radiation are complex and are regulated by a number of overlapping molecular pathways.One such stress-signaling pathway involves p53,which regulates the expression of over100genes already i-dentified.It is also becoming increasingly apparent that the pattern of stress gene expression has some cell type specifici-ty.It may be possible to exploit these differences in stress gene responsiveness as molecular markers through the use of a combined informatics and functional genomics approach.The techniques of microarray analysis potentially offer the op-portunity to monitor changes in gene expression across the entire set of expressed genes in a cell or organism.It again highlights the importance of a cellular context to genotoxic stress responses;it also raises the prospect of expression pro-filing of cell lines,tissues,and tumors.Such profiles may have a predictive value in cancer therapy regimens,or identifi-cation of exposures to environmental toxins.