烧伤大鼠血清对肠上皮细胞E-钙粘附蛋白表达的影响

Effects of Rat Burned Serum on the Expression of E-cadherin in Intestinal Epithelial Cell

目的 探讨烧伤血清对肠上皮细胞 E-钙粘附蛋白 (ECD)表达的影响及其意义。方法 培养大鼠肠上皮细胞株 IEC- 6 ;采用激光共聚焦及流式细胞术定量分析等技术 ,动态观察烧伤血清刺激培养的 IEC- 6细胞ECD的变化。结果 肠上皮细胞经烧伤血清作用后早期 ECD表达呈进行性下降 ,细胞通透性增加。结论 烧伤后肠上皮细胞细胞 ECD表达降低 。

Objective To explore the changes and significance of the intestinal epithelial E cadherin (ECD) following burn serum. Methods The intestinal epithelial cells (IEC 6) were cultured. The changes of ECD of the IEC were dynamically observed with laser scanning confocal microscopy (LSCM) and flow cytometry. Results In the early stage after stimulation by burn serum, the expression of ECD obviously decreased,and the permeability of IEC increased. Conclusion The expression of ECD in IEC was decreased, and the adhesion junction was damaged after burn injury.