FHIT基因与宫颈癌的相关性研究

Exploration of the Correlation Between FHIT Gene and Cervical Carcinoma

目的 探讨 FHIT基因在宫颈癌发生中的作用及与宫颈癌的临床分期和组织分化程度间的关系。方法 选择 FHIT基因的 2个微卫星多态标记对 4 2例宫颈癌进行杂合性丢失 (L OH)和微卫星不稳定性 (MI)分析。结果 在 D3S12 34和 D3S130 0座位上 ,L OH频率分别为 5 2 .5 % (2 1/ 4 0 )、70 .8% (17/ 2 4 ) ,MI频率分别为2 2 .5 % (9/ 4 0 )、2 0 .83% (5 / 2 4 )。FHIT基因改变与宫颈癌的临床分期和组织分化程度无关。结论 FHIT基因参与了宫颈癌的发生。

Objective To determine the role of fragile histidine traid (FHIT) gene in the development of cervical carcinoma and detect the relationship of FHIT gene with the clinical stage and tissue differentiation of cervical carcinoma. Methods Two sites of microsatellite polymorphism in FHIT were selected for detecting the loss of heterozygosity(LOH) and microsatellite instability(MI) in 42 samples of cervical carcinoma.Results In D3S1234 and D3S1300,the LOH frequencies were 52.5%(21/40) and 70.8%(17/24),while the MI frequencies were 22.5%(9/40) and 20.83%(5/24),respectively. No relationship of FHIT gene with the clinical stage and tissue differentiation of cervical carcinoma was observed. Conclusion FHIT gene participates in cervical carcinogenesis and may be one of the candidate tumor suppressor genes.Detecting the abnormal FHIT might be helpful to making a diagnosis of or screening for cervical carcinoma.