胰岛素样生长因子对少枝胶质细胞生长及髓鞘再生的影响

The effects of IGF-1 on the development of oligodendrocyte and remyelination in vitro

目的 观察胰岛素样生长因子 (Insulin likegrowthfactor 1,IGF 1)对离体培养的少枝胶质细胞、髓鞘生长及肿瘤坏死因子 (Tumornecrosisfactor α ,TNF α)脱髓鞘后的髓鞘再生的影响 ,以期为临床治疗多发性硬化 (Multiplescrosis ,MS)提供实验依据。 方法 采用体外器官型组织培养Wistar大鼠小脑组织的方法及免疫细胞化学方法 ,观察髓鞘形成率及表达 2’3’ 环核苷酸 3’ 磷酸二酯酶 (2’3’ cyclicnucleotide ,3’ phos phodiesterase,CNPase)阳性的少突胶质细胞数。 结果 IGF 1组及IGF 1+TNFα组髓鞘形成率明显高于TNF α组 (P <0 .0 1,P <0 .0 5 ) ,18天时髓鞘形成率分别为 90 %及 75 %。CNPase阳性细胞数IGF 1组及IGF 1+TNF α组明显高于TNF α组 (P <0 .0 1)。结论 IGF 1能促进少突胶质细胞及髓鞘生长 ,并能促进TNF α脱髓鞘后的髓鞘再生。

Objective It has been verified that cytokines play an important role in the immunological pathogenetic mechanisms of EAE and MS. We try to know the effects of TNF α on myelination and the development of oligodendrocyte directly. The organotypic culture of nerve tissue was used as a model of myelination and demyelination. In this study we sought to evaluate the implication of Inisulin like growth factor 1 (IGF 1) in the CNS myelination and in the repair of myelin that occur after a demyelinating process. It might provide the basis for approaches to promote remyelination.Methods Using organotypic cultures of wistar mouse cerebellum and immunocytochemical way, we studied the myelination rates and the numbers of CNPase positive oligodendrocyte.Results The myelination rate in TNF α group was affected significantly compared with the control group ( P < 0.01 ). The myelination rates in IGF 1 group and TNF α+IGF 1 group increased significantly compared with TNF α group ( P < 0.01 , P < 0.05 ). The number of CNPase positive oligodendrocyte in TNF α group was smaller than control ( P < 0.01 ), the numbers of CNPase positive oligodendrocyte in IGF 1 group and TNF α+IGF 1 group were larger than TNF a group ( P < 0.01 , P < 0.05 ).Conclusions TNF α can suppress the development of oligodendrocyte and myelination in vitro. IGF 1 can increase both the number of oligodendrocyte and amount of myelination and remyelination.