颈上神经节切除对豚鼠内淋巴囊血流量影响机制的研究

The Effoct of Superior Cervical Ganglion Resection on the Endolymphatic Sac Blood Flow

目的 探讨P物质 (substanceP ,SP)、降钙素基因相关肽 (calcitoningene -relatedpeptide ,CGRP)对内淋巴囊血流量及功能的影响 ,为了解某些内耳疾病的病因提供实验基础和理论依据。方法 建立颈上神经节切除模型 ,通过计算机图象分析计算SP、CGRP量的变化 ,检测内淋巴囊血流量。结果 颈上神经节切除后SP明显减少 (P <0 .0 1) ,CGRP无明显改变 ,内淋巴囊血流量明显减少 (P <0 .0 1)。结论 SP。

Objective To study the effect of substance P (SP), calcitonin gene-related peptide (CGRP) on the blood flow and the function of endolymphatic sac (ES) in guinea pigs to clarify the pathogenesis of some inner ear diseases further.Methods The guinea pig model of superior cervical ganglion (SCG) resection was set up. The ESs of the model animals were stained by SP and CGRP immunocytochemically. The staining images were analysed by the computer. The ESBF was measured with the biologic microphere method.Results Compared with the control group, the staining of SP decreased sharply in the model group, however, the staining of CGRP remained unchanged. In the model animals, the ESBF was decreased either.Conclusion SP and CGRP could take part in the regulation of ESBF to affect the function of ES further.