地高辛素标记探针原位杂交法检测B淋巴瘤CD23基因的表达

Detection of CD23 mRNA expression in B-cell lymphoma by in situ hybridization with digoxigenin-labelled DNA probe

目的 :研究B淋巴瘤CD2 3基因表达。方法 :SP免疫组化选出 2 0例B淋巴瘤并进行CD2 3基因蛋白(膜CD2 3)检测 ,地高辛素标记CD2 3DNA探针原位杂交检测CD2 3mRNA。结果 :膜CD2 3的阳性率 90 % ,CD2 3mRNA的阳性率 90 %。结论 :CD2 3在B淋巴瘤中高表达 ,这为研究CD2 3在B淋巴瘤中的发病机制和临床诊治提供了重要资料。

AIM: To detect CD23 mRNA expression in B-cell lymphoma. METHODS: 20 pathological diagnostic conformed B-cell lymphoma samples were selected. Surface CD23 protein expression was examined by SP-immunohistochemistry and CD23 mRNA was detected by in situ hybridization with digotoxin labeled CD23 DNA probe.RESULTS: The positive rates of both surface CD23 protein and CD23 mRNA expression in B-cell lymphoma samples were more than 90%. CONCLUSIONS: The high expression of CD23 was showed in B-cell lymphoma both in mRNA and in protein levels. The results of this study was useful for understanding the molecular mechanism of B-cell lymphoma and for clinical diagnosis and treatment of the disease.