HLA-DRB1等位基因多态性与食管癌遗传易感性研究

The genetic susceptibility of HLA-DRB1 alleles in esophageal neoplasm of Hubei Han Chinese

目的 :从基因水平探讨湖北地区汉族人食管癌HLA -DRB1等位基因的遗传易感性。方法 :运用序列特异性引物聚合酶链反应结合基因序列分析等技术 ,检测无亲缘关系湖北汉族健康人 136例、食管癌组 4 2例患者的HLA -DRB1等位基因。SAS统计软件数据处理。结果 :湖北地区汉族人食管癌患者与正常人比较 ,HLA -DRB1 0 90 1基因频率显著增高 (0 2 5 0 0vs 0 1397,P =0 0 2 8,OR =2 0 5 3,病因分数 =0 12 82 ) ;两者间其余HLA -DRB1等位基因分布频率差别均无显著。结论 :HLA -DRB1 0 90 1等位基因与湖北地区汉族人食管癌正相关 ,为其易感基因 ,该等位基因测序结果与其基因库第 2外显子序列吻合。

AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal neoplasm in Hubei Han Chinese. METHODS: HLA-DRB1 gene polymorphism in 42 patients with esophageal neoplasm and 136 normal control subjects was studied by PCR and sequence. RESULTS: Allele frequency of HLA-DRB1 *0901 allele was significantly higher in esophageal cancer patients than those in normal controls (0 2500 vs 0 1397,P =0 028; the odds ratio 2 053; etiologic fraction 0 1282).There were no association between the rested HLA-DRB1 alleles with patients. CONCLUSION: Individuals carrying HLA-DRB1 *0901 may be susceptible to esophagealo carcinoma, its nucleotide sepuence approachs to the corresponded allele sequence(exon 2)published in GenBank.