胃癌患者手术前后外周血自然杀伤细胞和T淋巴细胞亚群的变化

Changes of NK cell and T lymphocyte subsets in patients with gastric cancer before and after operation

目的 研究胃癌患者手术前后自然杀伤细胞 (NK细胞 )和T淋巴细胞亚群的动态变化。方法 采用单克隆抗体方法及MTT法检测45例胃癌患者手术前后NK细胞的活性及T淋巴细胞亚群的阳性率 ,并与40名健康体检者进行比较。结果 胃癌患者手术前NK细胞活性、CD3 +和CD4+细胞阳性率及CD4+/CD 8+比值均明显下降(均P<0.01) ,CD8+细胞阳性率升高(P<0.01)。术后28d ,患者细胞免疫功能逐渐恢复 ,与手术前相比差别均有显著性意义(P<0.05)。结论 胃癌患者细胞免疫功能与肿瘤的消长有密切关系。NK细胞活性和T淋巴细胞亚群的阳性率可作为疗效评定和预后评估的一个重要监测指标。

Objective To study the changes of nature killer cells(NK cell) and T lymphocyte subsets before and after operation of gastric cancer.Methods The T_cell subsets and NK cell activity of peripheral blood in 45 cases of gastric cancer were determined before and after operation.Forty normal subjects were used as the control group.In the rosette formation assay,anti_CD 3,CD 4,CD 8 monoclonal antibody_sensitized sheep red blood cells were used to detect T_cell subset,and the rate of rosette formation was considered as a percentage of CD 3+,CD 4+ and CD 8+ cells. The MTT colorimetric assay was used to detect NK cell activity.Results Before operation NK cell activity,CD 3+,CD 4+ cell and ratio of CD 4+/CD 8+ of gastric cancer patients were significantly lower and CD 8+ cell was higher as compared to control group (P<0.01).But these indicators were improved 28 days after operation as compared with those before operation (P<0.05).Conclusion Cellular immune function in patients with gastric cancer were improved after operation and T lymphocyte subsets and NK cell activity could be used as monitoring indicators.