单眼视网膜摘除鸽视盖差异表达基因的筛选

SCREENING OF THE DIFFERENTIALLY EXPRESSED GENES IN LEFT OPTIC TECTA OF PIGEONS WITH THEIR LEFT-RETINA REMOVED

目的 筛选单眼剥夺鸽视盖差异表达的基因。 方法 采用抑制消减杂交技术构建左侧视网膜剥夺后左视盖基因差异表达的文库 ,对部分重组克隆进行序列分析 ,反Northern进一步去除假阳性。 结果 随机测序 15 0个表达序列标签 ,16个表达序列标签经反Northern证实为真阳性 ,包括与基因库已知基因同源的及未发现明确的同源序列 ,138个基因片段已申请序列号。 结论 视盖差异表达基因的研究为深入研究视觉发育。

Objective To screen the differentially expressed genes in optic tecta of pigeon with monooccular deprivation. Methods Using suppression subtractive hybridization technique,the differentially expressed cDNA library for the left optic tecta of pigeons was constructed;recombinant clones were sequenced randomly and further proved to be the true positive by reverse Northern blot analysis. Results 150 of EST (expressed sequence tags) were sequenced and 16 of them proved true positive,which included those homologous to known genes and those having no matches.138 of ESTs were assigned Accession Number by Genbank.Conclusion\ Our data provided to be the useful clues for studying the development of visual system and the molecular construction of vision center system.