人甲状腺促性腺激素释放激素及其受体的表达

THE EXPRESSION OF GONADOTROPIN-RELEASING HORMOEE AND ITS RECEPTOR IN HUMAN THYROID GLAND

目的 探讨人甲状腺中是否存在促性腺激素释放激素 (GnRH)及促性腺激素释放激素受体 (GnRH R)并细胞定位 ,试图从转录及翻译水平讨论人甲状腺可否合成GnRH及GnRH R ,为探讨GnRH可能影响甲状腺的功能提供形态学依据。 方法 采用免疫组织化学法和原位杂交技术。 结果 所测人正常甲状腺组织均呈较强的GnRH和GnRH R免疫反应阳性 ,甲状腺滤泡上皮细胞、滤泡旁细胞均为阳性细胞。免疫反应阳性物质主要分布在阳性细胞胞质内 ,胞核呈阴性。原位杂交结果同免疫组织化学染色基本一致 :甲状腺滤泡上皮细胞胞质和滤泡旁细胞胞质可检测到较强的GnRHmRNA及GnRH RmRNA阳性杂交信号。胞核均呈阴性 ,未检测到杂交信号。 结论 人甲状腺不仅表达GnRH和GnRH R ,而且可以自身合成GnRH和GnRH R。

Objective To study whether gonadotropin releasing hormone (GnRH) and gonadotropin releasing hormone receptor(GnRH R) exist in human thyroid or not. Try to prove if GnRH and GnRH R can be produced by human thyroid at transcriptional and translational level and their cellular localization. Methods Immunohistochemical ABC method and in situ hybridization were used to study the expression and localization of GnRH and GnRH R in human thyroid. Results The human thyroid follicular epithelial cells and parafollicular cells showed positive immunoreactivity of GnRH and GnRH R. The reactive substance distributed in cytoplasm but not in nuclei of all positive cells. The result of in situ hybridization was identical with that of immunohistochemical ABC method. GnRH mRNA and GnRH R mRNA could be detected in the cytoplasm of thyroid follicular epithelial cells and parafollicular cells. And nuclei were negative yet.Conclusion GnRH and its receptor distribute in human thyroid. Thyroid can produce GnRH and GnRH R by itself. We inferred that GnRH might regulate the physiological function of thyroid by autocrine.