粉防己碱诱导人红白血病细胞凋亡的研究

STUDIES ON APOPTOSIS OF K_(562) CELLS INDUCED BY TETRANDRINE

目的 探讨粉防己碱诱导人红白血病细胞 (K56 2 )凋亡的作用。 方法 采用光镜、电镜、免疫荧光观察K56 2 细胞形态的改变 ,以流式细胞仪分析细胞周期 ,以ABC法检测细胞中Bcl 2和p5 3蛋白的改变 ,以TUNEL法检测细胞凋亡。 结果 K56 2 细胞经粉防己碱诱导 4 8h后 ,出现细胞凋亡早期形态学改变 ;流式细胞仪显示细胞DNA合成受到抑制 ;ABC法检测出细胞中Bcl 2水平降低和p5 3水平升高 ;TUNEL法原位检测揭示有DNA断裂。结论 粉防己碱可抑制K56 2 细胞的生长 ,其作用与浓度相关 。

Objective To study the effect of tetrandrine on K 562 cells and its possible mechanism. Methods Light microscope,electron microscope and immuno fluorescence staining were used to detect the morphological changes of K 562 cells,to analyse the cell cycle of K 562 cells FCM was performed,to detect the expression of Bcl 2 gene and wild type p53 gene in K 562 cells ABC method was carried out,and to detect in cell death TUNEL method was applied. Results K 562 cells treated with tetrandrine for 48?h showed early changes of apoptosis.DNA synthesis was reduced.Bcl 2 expression was decreased while wild type p53 gene expression was increased.TUNEL showed DNA breakage.Conclusion\ Tetrandrine inhibits the growth of K 562 cells.The effect is related with the concentration used.Tetrandrine can induce apoptosis of the cell.