交替三线性分解算法与反相高效液相色谱-二极管阵列检测方法相结合同时测定苯二酚的位置异构体

Simultaneous Determination of Isomers of Dihydroxybenzene Using Alternating Trilinear Decomposition Algorithm Combined with Reversed-Phase High Performance Liquid Chromatography/ Diode Array Detection

利用交替三线性分解算法与反相高效液相色谱 二极管阵列检测 (RP HPLC DAD)相结合 ,在色谱洗脱时间为1 0 86min～ 1 399min(间隔 1 / 1 50min)、紫外吸收波长为 2 68nm～ 2 98nm(间隔 1nm)时对苯二酚位置异构体的重叠色谱及光谱体系进行了分辨研究。分辨结果与实际结果一致。同时测定了水溶液中共存的邻苯二酚、间苯二酚和对苯二酚的含量 ,回收率分别为 (1 0 0 1± 1 0 ) % ,(99 4± 1 4) % ,(1 0 0 5± 1 7) %。研究结果表明 :该方法定量快速准确 ,实验操作步骤简单 ,解决了在干扰物存在条件下三者很难同时分辨的问题 ,说明三线性分解算法能有效地解决HPLC

Alternating trilinear decomposition (ATLD) algorithm is a promising method for second order calibration, which is based on an alternating least squares principle to overcome some shortcomings of the traditional parallel factor analysis (PARAFAC) algorithms, such as the sensitivity to the estimated component numbers and slow convergence. It utilizes an iterative procedure involving Moore Penrose generalized inverse computations based on singular value decomposition and can be applied to the simultaneous determination of several organic components even in the presence of unknown interfering components. The overlapped chromatogram and spectra system of dihydroxybenzenes were resolved by ATLD combined with reversed phase high performance liquid chromatography (RP HPLC)/diode array detection (DAD). The elution time was set from 1 086 min to 1 399 min with an interval of 1/150 min and the ultraviolet wavelength from 268 nm to 298 nm with an interval of 1 nm. The resolved results were in agreement with the actual results excellently. Catechol (CAT), resorcinol (RES) and hydroquinone (HYD) in aqueous solution were determined simultaneously, whose recoveries were (100 1±1 0)%, (99 4±1 4)% and (100 5±1 7)%, respectively. The results showed this experiment can be easily performed without resorting to time consuming and complicated procedures and quantified accurately. It successfully solved the resolving problem caused by chromatogram and spectra overlapping in the presence of interfering components. It also revealed the ATLD algorithm can be efficiently applied to solve the second order calibration problem in HPLC DAD.