过氧化氢酶荧光分析法及其在海洋水生生物酶活力测定中的应用

Studies on the Fluorometric Method for Determining Activity of Catalase and Its Application to Marine Biosamples

建立了一种简便、灵敏的测定过氧化氢酶的方法 .方法的线性范围为 1 .7× 1 0 - 3～ 1 .7× 1 0 - 2 U/m L,检测限 (LD=3 S0 /S)为 8.5× 1 0 - 4U/m L.将其用于海洋生物样品中过氧化氢酶活力的测定 。

A new promising method for determining activity of catalase and its application are presented. The measurement of catalase activity is based on the inhibition of catalase on Fenton reaction. Catalase catalyzes the dissociation of H 2O 2, one of the main reactants of Fenton reaction which yields ·OH stoichiometrically, and restrains the increment of the fluorescence intensity, produced as a result of quinoline hydroxylation. The decrease of fluorescence intensity was linearly related to the activity of catalase and the linear range covered from 1 7×10 -3 U/mL to 1 7×10 -2 U/mL. A detection limit of 8 5×10 -4 U/mL catalase was obtained. This method has been applied to the detection of catalase in marine biosamples and satisfactory results were obtained.