摘要
采用酶解法制备球毛壳原生质体 .确定酶解条件为 :菌龄 2 .5d ,渗稳剂为S/C .采用 5、15、15mg/mL的Novozym 2 34、蜗牛酶 ,溶壁酶混合酶液 ,30℃下酶解 1.5h .再生率可达 2 9.11% .研究了渗稳剂对原生质体制备与再生的影响 ,并对球毛壳原生质体的释放和再生过程进行了观察 .结果表明 :山梨醇可促进原生质体的释放和再生 ;Ca2 + 对原生质体的再生有促进作用 ;葡萄糖对再生菌株生长的促进作用优于山梨醇 .
Protoplasts of Chaetomium globosum were obtained successfully by using a mixed enzyme solution of 5 mg/mL of Novozym 234, 15 mg/mL of snailase and 15 mg/mL of lywallzym. The mycelia of 2.5 d old could be enzyme digested in 1.5 h at 30 ℃.The regeneration rate of protoplasts went up to 29% . Sorbital could improve the formation and regeneration of protoplasts while Ca 2+ improved regeneration only. Glucose improved the growth of regeneration colonies more effectively than sorbital. The protoplasts releasing and regenerating of Chaetomium globosum were observed during this study.
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
2002年第5期595-598,共4页
Journal of Harbin Institute of Technology
基金
国家自然科学基金资助项目 (5 0 1780 2 1)
