石歧杂鸡γ-干扰素基因的克隆与序列分析

Cloning and Sequencing Analysis of Interferon-γ from Shiqiza Chicken

根据现有鸡γ 干扰素基因序列设计引物 ,应用反转录 聚合酶链反应 (RT PCR)技术 ,从ConA诱导培养的鸡脾淋巴细胞中扩增得到石岐杂鸡γ 干扰素 (sqzChIFN γ)基因 ,将其克隆到pGEM T载体中 ,进行序列测定 .结果表明 ,克隆得到的ChIFN γ基因的开放阅读框架由 4 92个核苷酸组成 ,和其他ChIFN γ基因的同源性达 99% ,与火鸡γ 干扰素基因的同源性为 96 % ,与鸭γ 干扰素基因的同源性为 81% .推导的石岐杂鸡IFN γ氨基酸序列与其他已发表的ChIFN γ氨基酸序列完全一致 ,与火鸡和鸭IFN γ氨基酸同源性分别为 97%和 6 7% .

The Shiqiza chicken interferon γ(sqzChIFN γ)was amplified by reverse transcription polymerase chain reaction(RT PCR) from splenocytes stimulated with Con A. The gene was cloned into pGEM T vector and then sequenced. The result showed that the open reading frame of cloned ChIFN γ consisted of 492 nucleotides, sharing 99% homology with other ChIFN γ gene, 96% homology with turkey IFN γ and 81% with duck IFN γ. The deduced amino acid sequence of sqzChIFN γ was completely identical to other published sequence of ChIFN γ,and shared 97%and 67% homology with turkey and duck IFN γ, respectively.