摘要
目的 :通过体外实验 ,研究肺炎链球菌 (Streotococcus pneumoniae,S.pn)是否可触发肺 型上皮细胞 (A5 49)胞浆 [Ca2 +] i 浓度的改变。方法 :用 F ura-2 /AM荧光探针负载 A5 49细胞后测定 S.pn粘附A5 49细胞 3 0、60、90 min的胞内 [Ca2 +] i 浓度。结果 :S.pn粘附 A5 49细胞 3 0、60、90 min后的胞内 [Ca2 +] i均高于对照 [(187.4± 17.3 ) nmol/L ] ,并达到饱和 ,分别为 (4 87.5± 3 8.1)、(5 48.2± 3 5 .6)、(5 5 7.2± 47.5 )nmol/L。结论 :上述结果提示 S.pn粘附 A5 49细胞可增加胞浆内 [Ca2 +] i
Objective:To investigate whether Streptococcus pneumoniae(S.pn) can increase the concentration of free calcium in type Ⅱ pneumocytes(A549 cells) in vitro. Method:A549 cells were loaded with Fura 2/AM and the concentrations of free calcium were determined during 30?60?90 minutes respectively.Result:The concentrations of free calcium during 30?60?90 minutes are all higher than that of control group.They are(487 5±38 1)?(548 2±35 6)?(557 4±47 5)nmol/L respectively.Conclusion:The concentration of free calcium in A549 cells are increased after the adhesion of S.pn to A549 cells.
出处
《中国微生态学杂志》
CAS
CSCD
2002年第5期254-255,共2页
Chinese Journal of Microecology
