神经降压素对失血—感染大鼠肠道细菌易位的实验研究

EFFECT OF NEUROTENSIN ON INTESTINAL BACTERIAL TRANSLOCATION IN THE RATS SUBJECTED TO HEMORRHAGE-INFECTION

目的 :探讨神经降压素 (Neurotensin,NT)对肠道细菌易位 (Bacterialtranslocation,BT)的影响。方法 :80只 Wistar大鼠随机分成假手术组、单纯失血组、单纯感染组、失血—感染组及 NT1 、NT2 、NT3 7个组 ,NT组均先制成失血—感染模型 ,然后分别用 NT 10μg/kg、NT 3 0μg/kg、NT3 90μg/kg皮下注射 ,3 h后取各组肝、肠系膜淋巴结 (mesenteric L ymph node,ML N)制成匀浆进行细菌培养 ,抽门静脉血测定血内毒素水平 ,7组均取未段回肠 1cm进行光镜和电镜检查。结果 :除 NT1 外 ,失血—感染组 BT率、组织细菌含量、血内毒素水平均显著高于其他各组 ,有统计学差异。NT3 组 BT率、组织细菌含量、血内毒素水平均最低 ;病理结果显示 :NT1 、NT2 、NT3 组肠粘膜受损伤较单纯失血、单纯感染、失血感染组均轻 ,有统计学差异。结论 :NT能加速损伤肠粘膜修复 ,增强小肠粘膜屏障功能 ,阻止细菌易位 。

Objective:To study the effect of neurotensin(NT) on intestinal bacterial translocation(BT) in rats after hemorrhag infection.Methods:Wistar rats were divided randomly into 7 groups,namely,sham operated group,hemorrgahe group,infection group,hemorrhage infection group,NT1 group,NT2 group and NT3 group Different doses of neurotensin(10?30?90 μg/kg)or saline were injected subcutaneously to rats subjected to hemorrhage infection or controls.After 3 hours,the bacterial cultures of mesenteric lymph node(MLN) and liver were performed together with the measurement of portal endotoxin(ET) level in all groups.Moreover,the degrees of distal ileal mucosa damage are classified according to light and electronic microsocope.Result:The average number of bacterial translocation to liver and MLN(3 94±2 35,7 01±3 72)and ET level (0 883±0 282)were significantly higher respectively in hemorrhage infection group than that in other groups,they were statistic significance, P <0 01,Except NT1,the rate of BT in hemorrhage infection group was the highest among all groups.The rate of BT(16 7%),the average number of bacterial translocation to liver and MIN(1 67±1 03,2 01±0 9)and ET level(0 026±0 017) in NT3 group were the lowest among all groups.Under light and electronic microscope,incomparision with hemorrhage infection group,intestinal mucosa damage in NT groups were more slightly.Conclutions:NT can enhance intestinal barrier function and quickly heal intestial mucosa subjected to damage,further,to prevent bacterial translocation.NT prevents bacterial translocation relation its dose