p53基因与顺铂联合作用对人卵巢癌细胞的杀伤效果

Efficacy of cisplatin against human ovarian cancer cells when combined with p53 gene therapy

目的 :探讨外源性p5 3基因转染对人卵巢癌细胞系SKOV 3的生物学行为影响及与顺铂联合作用后对人卵巢癌细胞的杀伤效果。方法 :用脂质体介导的转染技术 ,将p5 3基因的真核细胞表达载体 (PcDNA p5 3)导入不表达p5 3的人卵巢癌SKOV 3细胞中 ,经G4 18筛选 ,Northernblot及Westernblot鉴定后 ,观察其对细胞生物学行为的影响及与顺铂联合作用后对细胞集落形成的影响。结果 :转染后获得稳定表达p5 3的转染克隆。外源性p5 3基因的表达明显抑制了卵巢癌细胞的生长及集落形成能力 ,使细胞阻滞于G1 期 ,并增加了卵巢癌细胞对顺铂的敏感性。结论 :外源性野生型p5 3基因能抑制卵巢癌细胞的生长 ,与顺铂联合作用后增强了对人卵巢癌细胞的杀伤效果。

Objective:To explore the effects of wild type p53 gene on biologic behavior of human ovarian cancer cell line SKOV 3 and the efficacy of cisplatin against the cancer cells when combined with p53 gene therapy.Methods:A human wild type p53 gene recombinant eukaryotic expression vector was introduced by lipofectin mediated gene transfection into SKOV 3 cells which does not express endogenous p53.The clones obtained were observed for their biological behavior and the colony formation when exposed to cisplatin.Results:The exogenous wild type p53 gene expressed stably in the cells.The growth rate and colony formation of these transfected cells were suppressed significantly by exogenous p53 gene and the percentage of phase G 1 cells increased.There was an increasement in the sensitivity to cisplatin of the transfected cells.Conclusion:Exogenous wild type p53 gene can suppress the growth of human ovarian cancer cells and increase the efficacy of cisplatin against the cells.