人类脐血清的抗补体效应

Anticomplement effect of cord blood serum

目的 :探讨在人类脐血清补体的抑制效应。方法 :将经典补体溶血实验进行改进 ,在原有反应体系中加入脐血清 ,测定并计算血清抑制补体活性的百分抑制率 (Inhibition % ) ,Inhibition %均以 x±s表示。结果 :(1)脐血清Inhibition % (32 77± 11 2 9) %比正常成人 (15 0 7± 4 6 6 ) %明显升高。两者相比差别显著 (P <0 0 1) ;(2 )正常孕妇分娩前血清 (15 79± 6 0 9) %与其胎儿脐血清 (33 98± 11 94 ) %抗补体作用有明显差异 (P <0 0 1)。结论 :脐血清有较强的抗补体作用 ,可能在减弱全脐血移植时由补体介导的急性移植排斥效应反应的发生发展中起一定作用 ,为全脐血移植疗法的好效果提供了免疫学方面的新解释。

Objective:To investigate anticomplement effect of cord blood serum.Methods:Based on the classical complement hamolytic assay,added serum in the reactions,then took the percentage of complement inhibited(Inhibition%) as the level of anticomplement effect of serum.Results:(1)Anticomplement activity in serum of cord blood(32.77±11.29)% was significantly higher than of maternal serum(15.07±4.66)%(P<0.01).(2)The sera of the normal antepartal pregnant women show lower inhibition%(15.79±6.09)% than those of cord blood of their infants(33.98±11.94)%(P<0.01).Conclusion:It seemed that significantly anticomplement effect of cord blood serum may prevent host versus graft reaction,which frequently occurred after transplantation.