一种新强心苷类化合物的结构鉴定及其活性研究(英文)

Structure elucidation and activity evaluation of a new cardenolide from Tupistra wattii Hook.f.

目的从弯蕊开口箭 (TupistrawattiiHook .f.)中寻找抗癌活性先导化合物。 方法使用稻瘟霉活性筛选体系进行活性追踪分离 ,并通过波谱分析 ,特别是二维核磁手段结合化学方法对分离得到的化合物进行结构鉴定。结果从弯蕊开口箭中分离得到 3个具有很强细胞毒活性的强心苷 ,其中化合物 2wattosideF是一个新化合物 ,并且它的细胞毒活性在 3个强心苷中最强 ,其IC50 值为 0 0 11μmol/L。 结论wattosideF是一个具有很强细胞毒活性的新的强心苷类化合物。

Aim To screen for cytotoxic compounds from Tupistra wattii Hook.f.Methods The isolation was guided by the Pyricularia oryzae bioassay,and the structures of the compounds were confirmed on the basis of the spectroscopic analysis and chemical evidence.Results Three cytotoxic cardenolides,rhodexin A[sarmentogenin 3 O α L rhamnopyranoside](1),wattoside F[sarmentogenin 3 O β D glucopyranosyl(1→2) α L rhamnopyranoside](2)and wattoside E[oleandrigenin 3 O β D glucopyranosyl(1→2) α L rhamnopyranoside](3)were isolated from the fresh rhizoma of Tupistra wattii Hook.f.Among the three compounds,Wattoside F was a new compound,and showed the strongest cytotoxicity against the cancer cell line K562 in vitro ,with the IC 50 value of 0 011 μmol/L.Conclusion Wattoside F was a new cytotoxic cardenolide from Tupistra wattii Hook.f.