大鼠胃肠道术后肠道相关淋巴组织的变化及益生体的作用

Alterations in Peyer's patches lymphocytes following surgery/antibiotics administration and the effects of probiotics in rats

目的观察手术、抗生素对淋巴集结 (Peyer′spatches)中淋巴细胞亚群和肠道微生态的影响 ,并观察益生体对机体的保护作用。方法SD大鼠分空白对照组、手术组、抗生素组和治疗组 4组 ,分别施行肠切除和胃造瘘 ,术后在后 2组应用抗生素 ,并在治疗组加用乳酸菌 ,喂养 7d。结果(1)手术及术后应用抗生素明显降低了Peyer′spatches中淋巴细胞总数及B细胞的数量 ;T细胞及其中CD4+ T细胞的变化不如B细胞及CD8+ T细胞显著。 (2 )手术及抗生素破坏了肠道菌群的平衡 ,主要表现为肠道细菌总量、厌氧菌及大肠杆菌量的减少。 (3)肠道细菌的变化与Peyer′spatches中淋巴细胞的变化有相同趋势。 (4 )新一代益生体Lb .Plantarum 2 99v防止了Peyer′spatches中淋巴细胞的破坏。结论 (1)手术或抗生素明显损害了肠道相关淋巴组织功能。 (2 )微生态的破坏可能与免疫功能下降相关。 (3)目前的常规肠内营养配方中均不含益生体 。

ObjectiveThis study was to investigate the effects of surgery and antibiotics on sub-type of lymphocytes and bowel microecology, and the possible beneficial effects of probiotics in protecting gut associated lymphoid tissue(GALT). Methods Male SD rats were subjected to receive gastrostomy and small bowel resection with or without treatment of antibiotics or antibiotics plus lactobacillus plantarum 299v postoperatively for a week. Each group consists of 6 rats. ResultsSurgery and antibiotics significantly reduced the total amount of lymphocytes especially the B cell and CD8 +T cell subtypes in Peyer′s patches(PP), and destroyed the microflora balance. Probiotics protect PP lymphocytes. Conclusions This study found the alteration in lymphocytes sub-types in PP following GI surgery and antibiotics administration. Probiotics has protective effects on GALT composition.