细胞核仁形成区酸性非组蛋白在胆囊癌诊断与监测中的价值

Value of Ag-NORs measurement in the diagnosis of patients with gallbladder carcinoma

目的探讨检测细胞核仁形成区酸性非组蛋白在胆囊癌诊断与监测中的价值。方法应用细胞银染技术对体外激活的外周血和胆汁淋巴细胞染色 ,并对胆囊组织作核仁形成区嗜银蛋白银染 ,计算外周血和胆汁中硝酸银染色酸性非组蛋白 (Ag) NORs面积与核面积的比值 (IS % )和组织切片中AgNOR颗粒面积。结果正常人 (2 0例 )、慢性胆囊炎 (90例 )与胆囊癌之间 (2 1例 )外周血、胆汁T细胞Ag NORs面积与核面积的比值和组织切片中AgNOR面积差异均有显著性意义 ,血清、胆汁中T细胞Ag NORs面积与核面积的比值 (IS % )逐级降低 ,组织切片中AgNOR面积逐级升高 ,且血清、胆汁中T细胞Ag NORs面积与核面积的比值 (IS % )、组织中AgNOR面积三者相互间有良好的直线相关性 (P均 <0 0 1)。结论监测胆囊病变过程中患者外周血、胆汁中T细胞Ag NORs面积与核面积的比值 (IS % )和组织切片中AgNOR面积的变化 。

ObjectiveTo evaluate the changes of Ag-NORs in patients with gallbladder carcinoma.MethodsAg-NORs in the peripheral blood lymphocytes,bile lymphocytes and the mucosa of gallbladder were detected by a cell image analysis of KL-2 in 20 normal volunteers,90 cholecystitis cases, and 20 gallbladder carcinomas. ResultsAg-NORs in peripheral blood lymphocytes (PBL) and bile lymphocytes (BL) in cholecystitis were lower than that in normal group(P<0 01),but higher than in cases of carcinomas(P<0 01),the expression rates of the Ag-NOR in gallbladder mucosa in cholecystitis were higher than that in the normal group(P<0 01), lower than in the carcinoma group(P<0 01). Ag-NORs in PBLs and BLs decreased and Ag-NOR in the mucosa of gallbladder increased steadily alongside the changes of gallbladder mucosa from hyperplasia, dysplasia to carcinoma. There was a linear relationship between the Ag-NORs in PBLs, BLs and the mucosa of gallbladder(P<0 01).ConclusionAg-NORs in PBLs, BLs and the mucosa of gallbladder may well be a new tumor marker for gallbladder carcinoma.