乳腺癌患者骨髓微转移细胞表型及骨髓血u-PA活性的研究

Phenotype profiles and u-PA activity of bone marrow micrometastasis in breast cancer patients

目的通过检测乳腺癌患者骨髓微转移细胞表型 ,研究骨髓微环境。方法采用免疫组化染色检测乳腺癌患者骨髓中肿瘤细胞的生物学标志及骨髓血浆的u PA活性。结果 72例乳腺癌患者中 ,30例 (4 1 7% )存在骨髓微小转移灶。其转移率与原发灶肿块大小 (χ2 =6 417P =0 0 40 )、p5 3蛋白表达 (χ2 =5 930 ,P =0 0 2 5 )相关。与原发灶肿瘤相比 ,肿瘤转移细胞的CyclinD1、p5 3、Ki 6 7、EGFR蛋白低表达 ,而p2 1蛋白高表达。骨髓血浆u PA活性与肿块大小、腋淋巴结状况密切相关。结论 骨髓中播散肿瘤细胞处于低生长。

Objective To study the phenotype profiles of bone marrow micrometastatic tumor cells in patients with breast cancer. Methods Immunocytochemical staining was used to study the biologic markers of disseminated tumor cells, and U-PA activity of the plasma in the bone marrow was assayed in breast cancer patients.Results Judged by positive immunocytochemical staining of EMA and CK19, bone marrow micrometastasis was found in 30 out of 72 (41 67%) breast cancer patients. The primary tumor size (χ 2=6 417, P=0 040) and p53 protein expression (χ 2 =5 930, P=0 025) were significantly correlated with the incidence of micrometastasis. Disseminated tumor cells expressed low protein cyclinD1, p53,Ki-67, EGFR, and high protein p21. Tumor size and axillary lymph node status were found to be significantly correlated with the u-PA activity level. Conclusion Disseminated tumor cells in the bone marrow was in a status of low-cycling and low-proliferation.