脑牵拉引起脑缺血性损伤的实验研究

Experimental Study of Brain Ischemic Injury Induced by Brain Retraction

目的 探讨脑牵拉压(BRP)的测量方法,研究脑牵拉引起脑缺血性损伤的危险性。方法 利用电阻应变计制作脑牵拉压的测量装置,对其定标。然后利用此装置,选用新西兰大耳白兔12只,分为对照组和20mmHg组、30mmHg组、40mmHg组、牵拉完毕后,测定局部脑血流量,标本HE染色显微镜观察其损伤。结果 该装置具有很高的灵敏性、稳定性和准确性。20mmHg的BRP牵拉30min引起轻微脑损伤和脑血流量的降低,30mmHg的BRP牵拉30min引起较重脑损伤和脑血流量的显著降低。40mmHg的BRP牵拉15min引起严重脑损伤和脑血流量的极度降低。结论 该装置可作为脑牵拉压的测量工具;牵拉时,最好将BRP控制在30mmHg以下。

Objective To investigate the method of measurement of brain retraction pressure (BRP) and observe the risks of brain ischemic injury induced by brain retraction. Methods Twelve rabbits were randomly divided into control, 20 mmHg (the brain retracted by 20 rnmHg pressure), 30 mmHg (by 30 mmHg pressure), 40 mmHg (by 40 mmHg pressure) groups. BRP was determined by self-made BRP instrument . The regional cerebral blood flow (rCBF) was measured. The specimen taken from brain tissues retracted by different BPR were observed under microscope. Results The mild brain injuries and slight decrease in the rCBF after lasting 30min retraction were observed in 20 mmHg and 30 mmHg groups. The severe brain injuries and significant decrease in the rCBF could be seen after retraction lasting 15min in 40 mmHg groups. Conclusions This instrument can be used as a tool for measuring BRP. The BRP during the brain surgery should be not more than 30 mmHg to avoid brain injuries induced by the retraction.