大鼠皮肤创伤后EⅢA-FN mRNA表达与损伤时间推断研究

Expression of EⅢA-fibronectin in injured rat skin used in estimation of wound interval

目的研究皮肤创伤后不同时间纤连蛋白剪接方式变化及EⅢA剪接异型体的表达。方法提取伤区组织总RNA,RT-PCR方法扩增目的条带。结果大鼠皮肤损伤后表达出正常组织中没有的EⅢA+(526bp)片段,其中伤后1h该条带肉眼可辨认,在伤后24h内,随损伤时间延长其条带亮度相应升高。结论EⅢA-FN剪接异型体表达变化可为法医学损伤时间推断较理想指标。

Objective To observe the means of fibronectin(FN)alternative splicing and the expression of E Ⅲ A-FN variant in rat skin after bruise,for the sake of providing some help for forensic estimation of wound interval.Methods Total RNA was isolated from wounded skin,and reverse transcription polymerase chain reaction was conducted to amplify target segments.Results Detectable E Ⅲ A + (526bp)segments,lacked in normal organize,was amplified at1h after experimental wound,and the levels were increased within24h.Conclusion The alter native splicing E Ⅲ A-fibronectin variant would be a satisfied criterion for research of skin injury.