离体人胸骨骨髓DNA降解与腐败尸体死亡时间推断的初步研究

The relationship between postmortem degradation of marrow DNA in bosom bone and late postmortem interval estimation

目的研究死后胸骨骨髓DNA的降解与较长死后间隔时间(PMI)的关系。方法采用改良Feulgen染色及计算机图像分析技术对离体人胸骨(取材后分别室温搁置0,1,3,5,7d)骨髓DNA含量进行定量检测。结果在较长PMI范围内,人胸骨骨髓DNA含量仍呈现下降规律。结论人胸骨骨髓DNA降解规律可望应用于较长PMI的推断。

Objective To study the relationship between degradation of marrow DNA and late postmortem interval(PMI).Methods Marrow were left on natural condition for0,1,3,5,7day after death respectively,Marrow DNA were detected by using Feulgen staining and computerized image analysis.Results The content of marrow DNA could be detected till7days afer death yet.Conclusion The degradation of marrow DNA may be used on estimation the late PMI.