摘要
目的 研究多发性骨髓瘤 (MM)细胞系培养上清液对人骨髓来源的内皮细胞系(HBMEC)的增殖、迁移、血管新生的促进作用及酞咪哌啶酮对其抑制作用。方法 用MM细胞系 (IM9、XG1、U2 6 6和MOLP 5 )培养上清液培养HBMEC ,检测其对HBMEC的增殖、迁移作用。在纤维蛋白胶及基质胶 (matrigel)中观察此上清液对血管新生的影响 ,并研究酞咪哌啶酮对这些作用的抑制。用酶联免疫吸附实验 (ELISA)测定此上清液中血管内皮生长因子 (VEGF)含量。结果 MM培养上清液明显促进HBMEC的增殖及迁移。在纤维蛋白胶及基质胶 (matrigel)中 ,促进微血管的生长。这些作用均可被酞咪哌啶酮抑制。所有这些作用与MM细胞系产生的VEGF无相关性。结论 MM细胞系通过分泌VEGF或其他某些细胞因子促进HBMEC的增殖、迁移及微血管形成 ;酞咪哌啶酮可抑制这些作用 。
Objective To investigate the pro-angiogenic effects of several multiple myeloma(MM) cell line culture supernatants on human bone marrow endothelial cell (HBMEC) proliferation, migration, and capillary formation, and the anti-angiogenic effects of thalidomide. Methods HBMEC was cultured in the presence of MM cell lines (IM9, XG1, U266 and MOLP-5) supernatants. Proliferation and migration of HBMEC were determined, capillary-like tubule formation of HBMEC was examined in fibrin and Matrigel. The inhibiting effect of thalidomide was investigated by adding it into myeloma cell line culture supernatants. Vascular endothelial growth factor (VEGF) was measured by ELISA. Results ① MM cell lines culture supernatants promoted HBMEC proliferation and migration. ②In fibrin and Matrigel, capillary-like tubule network formation promoted by the supernatants. ③All of these effects could be inhibited by thalidomide. ④ This effect was not related to VEGF in the supernatants.Conclusions MM cell line promote proliferation, migration and tubule formation by secreting VEGF or other several cytokines. Thalidomide can inhibit these effects.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2002年第10期520-523,共4页
Chinese Journal of Hematology
基金
北京市科技新星计划基金项目部分资助 ( 95 4812 30 0 )
