细胞因子基因多态性与细胞因子水平关系的研究

Study on the relationship between cytokine gene polymorphisms and their serum levels

目的 探讨细胞因子基因多态性与其血清水平的关系。方法 随机选取 65名汉族健康成人 ,分析其肿瘤坏死因子 α(TNF α)、转化生长因子 β1 (TGF β1 )和白细胞介素 1 0 (IL 1 0 )基因型及分泌水平。细胞因子基因型检测采用序列特异引物聚合酶链反应 (PCR SSP)法 ,血清细胞因子水平测定采用ABC ELISA法。结果 TNF α等位基因型别共有 3种 ,分别为A/A、G/A和G/G ,对应的血清TNF α浓度 ,A/A组和G/A组均比G/G组高 (P <0 .0 1 ) ;TGF β1等位基因型别共有 9种 ,分别为T/T G/G、T/C G/G、T/C G/C、C/C G/G、T/T G/C、C/C G/C、C/C C/C、T/T C/C和T/C C/C ,对应的血清TGF β1浓度 ,T/T G/G、T/C G/G组比T/C G/C、C/C G/G和T/T G/C组高 (P <0 .0 1 ) ,而T/C G/C、C/C G/G和T/T G/C组又比C/C G/C、C/C C/C、T/T C/C和T/C C/C组高 (P<0 .0 1 ) ;IL 1 0等位基因型别共有 6种 ,分别为GCC/GCC、GCC/ACC、GCC/ATA、ACC/ACC、ACC/ATA和ATA/ATA ,对应的血清IL 1 0浓度 ,GCC/GCC组比GCC/ACC和GCC/ATA组高 (P <0 .0 1 ) ,而GCC/ACC和GCC/ATA组比ACC/ACC、ACC/ATA和ATA/ATA组高 (P <0 .0 1 )。结论 在受测人群中 。

Objective To investigate the relationship between cytokine gene polymorphisms and their serum levels. Methods 65 healthy adult Han people were chosen randomly and were divided into groups according to their cytokine genotypes of tumor necrosis factor α (TNF α),transforming growth factor β1 (TGF β1) or interleukin 10 (IL 10). The cytokine genotypes were detected by PCR SSP method. The serum levels of cytokines were assayed by ABC ELISA. Results The serum level of TNF α in the group with the TNF α high producer genotype was higher than that with the TNF α low producer genotype( P < 0.01 ).Similarly,the serum level of TGF β1 in the group with the TGF β1 high producer genotype was higher than that with the TGF β1 low producer genotype ( P < 0.01 ).The serum levels of TGF β1 in the group with the TGF β1 low producer genotype were lower than those in the group with the TGF β1 mediate producer genotype( P < 0.01 ). The same relationship about IL 10 genotypes and serum levels was found.Conclusion TNF α,TGF β1 or IL 10 genotypes are determinants of their corresponding serum levels in healthy adult Han people.