有机荧光染料纳米颗粒的制备及其包埋机制的研究

Preparation of organic fluorescent dye doped nanoparticle and research of encapsulated mechanism

该文报道了用油包水的微乳液方法,以三种水溶性的荧光染料:异硫氰酸荧光素(FITC)、标记葡聚糖(分子量为282000)的异硫氰酸荧光素(FITC-dextran)与连有免疫球蛋白IgG(分子量为 156000)的异硫氰酸荧光素(FITC-IgG)为核材料,二氧化硅为外壳形成了核壳结构的纳米颗粒。通过考察三种染料在壳层中的泄漏情况,发现FITC-IgG只有50％的泄露,而FITC-dextran与FITC几乎全部泄露,同时二氧化硅壳层的越厚,染料越不易漏出。

We have prepared core - shell nanoparticles using three fluorescent dyes as core materials, that is fluorescein isothiocyante (FITC), FITC conjugated dextran (MW=282000) and FITC conjugated Goat anti - Humane immunoglobulin (IgG) (MW = 156 000) and silica as shell material with a reverse microemulsion technique. The FITC encapsulation efficiency is greatly enhanced conjucting a water - soluble protein IgG which significantly reduces FITC leaching from the nanoparticles. We also find that the thicker layer the silica nanoparticle has, the less dye leaching from the nanoparticle will occur.