非水溶性的β-环糊精聚合物的主客体包合作用及在过氧化氢生物传感器中的应用

Host - guest inclusion of insoluble β- cyclodextrin Polymer and Its Application to Hydrogen Peroxide Biosensor

非水溶性的β-环糊精交联聚合物(β-CDP)与戊二醛缩聚生成的聚合物带有悬挂的羰基,使辣根过氧化酶共价固定化;β-CDP与介体甲苯胺兰(TB)发生主客体包合作用将介体固定在电极表面,既避免了介体的流失又保证了介体有充分的流动性,实现了介体和酶的同时固定化。由于包络物的形成,使得H_2O_2生物传感器有较好的稳定性及较高的灵敏度,其线性相应范围为5.0μmol/L～10mmol/L,检测限为1μmol/L,达95％稳态响应电流所用时间少于30s。

In this paper, insoluble β- CD polymer(β- CDP) with glutaric dialdehyde forms the polymer with pending carbonyl, the latter brings about covalent immobilisation of horderadish peroxidase(HRP). Because the formation of supermolecular complex between β - GDP and mediator toluidine blue(TB), TB is prevented from flow away, the stability and the life time of the biosensor are improved greatly. The linear response of the H2O2 biosensor ranges is from 5. 0 μmol/L to 10 mmol/L with detection limit of 1. 0 μmol/L,and the 95% steady - state response time is less than 30 s.