外源性IL-10对炎症牙龈组织中IL-6和ICAM-1表达的影响

Effects of Exogenous IL-10 on IL-6 and ICAM-1 Expression in Inflammatory Gingival Tissue

目的 :探讨外源性IL_10对炎症牙龈组织中IL_6和细胞间粘附分子_1(ICAM_1)表达的影响。方法 :采用免疫组化技术检测牙周袋内用或不用IL_10时成人牙周炎患者牙龈组织中IL_6和ICAM_1的表达。结果 :IL_6和ICAM_1均可在炎症牙龈组织的多种细胞表达。IL_6主要表达于淋巴细胞、单核—巨噬细胞、成纤维细胞和内皮细胞。I CAM_1主要表达于淋巴细胞、上皮细胞、单核—巨噬细胞、成纤维细胞和内皮细胞。使用Image_Proplus软件系统测得的IL_6和ICAM_1表达的IOD值在实验组和对照组间有显著性差异 (P <0 0 5 )。结论

Objective:This study aimed at investigating effects of exogenous interleukine-10 (IL-10) on IL-6 and intercellular adhesion molecular (ICAM-1) expression in inflamed gingival tissue.Methods: The expression of IL-6 and ICAM-1 was examined using immunohistochemical techniques. Inflammatory gingival tissue treated with IL-10 was taken as the experimental group and the same patient's inflammatory gingival tissue without treatment of IL-10 was included into the control group.Results:IL-6 expression was found mainly in monocytes, macrophages, lymphocytes, endotheliocytes and fibroblasts. The expression of ICAM-1 was found mainly in epithelial cells, monocot-macrophages, lymphocytes, endotheliocytes and fibroblasts. The immunohistochemical optical density (IOD) of the expression of IL-6 and ICAM-1 was detected by using Image-Proplus software, and the results showed that the expression in the experimental group differed significantly from that in the control group.Conclusion:The exogenous IL-10 may down-regulate IL-6 and ICAM-1 expression in inflammatory gingival tissue.