培养的钙化大鼠心肌细胞牛磺酸转运障碍

An Observation of Taurine Transport Alterations in Calcification of Myocardial Cells in vitro

目的探讨离体培养钙化幼鼠心肌细胞牛磺酸(Tau)跨膜转运功能及牛磺酸转运体(TAUT)和半胱亚磺酸脱羧酶(CSD)mRNA含量的改变及意义。方法以β-磷酸甘油培养心肌细胞;氨基酸分析仪测定细胞牛磺酸含量;3H标记的牛磺酸测定心肌细胞牛磺酸转运;竞争性定量RT-PCR测定心肌细胞TAUT和CSDmRNA水平。结果与非钙化大鼠心肌细胞相比,钙化心肌细胞牛磺酸含量减少27%(P<0.05),细胞3H-牛磺酸摄入程度降低,最大转运速率(Vmax)减少39%(P<0.01),Km值两组间差异无显著性(P>0.05);钙化心肌细胞TAUTmRNA含量减少45%(P<0.01),而CSDmRNA增加25%(P<0.05)。结论钙化心肌细胞牛磺酸转运功能障碍,TAUT表达减少,而CSD表达增加。

Objective To observe the alterations of taurine transport,taurine transporter(TAUT)and cysteine sulfinate decarboxylase(CSD)mRNA in the calcification of myocardial cells in vitro.Methods 3 H-taurine measured the amount of taurine uptake.TAUT and CSD mRNA concents were measured using competitive quantitative RT-PCR in cultured and calcified myocardial cells.Results In calcification of myocardial cells,taurine concentration was decreased by27%(P<0.05),taurine uptake was markedly reduced,Vmax reducedd by39%(P<0.01),there were no statistical significance of Km values between the two groups.TAUT mRNA decreased by45%(P<0.01),but CSD mRNA increased by25%(P<0.05).Conclusions The data suggest that there were impediment of taurine transport in calcification of myocardial cells,as TAUT mRNA level was decreased,but CSD mRNA concentration was improved.