北京地区尖锐湿疣患者皮损中人乳头瘤病毒的检测和分型

Identification and Assessment of Multiple Human Papillomavirus Types in Condyloma Acuminata Lesions from Patients with Genital Warts in Beijing Area

目的分析北京地区尖锐湿疣患者人乳头瘤病毒的型别和发病特点,比较普通患者和抵抗力弱的患者的发病差异。方法采用PCR和RFLP方法分析HPV型别,对于难以通过酶切鉴定的PCR产物,构建重组测序质粒,检索GenBank进一步分型鉴定。结果普通患者HPV6型为主要感染型别,其次是HPV11型。HPV6型患者年龄较HPV11和HPV6+HPV11型更低;而高龄患者和免疫抑制患者则以HPV11+HPV6的混合感染和HPV11感染为主。部分患者还检测出HPV16和HPV53感染。结论HPV6是尖锐湿疣的主要致病型别,但患者年龄比其他型别更低,而抵抗力弱的患者则多与HPV11的感染有关。一般存在于正常生殖道组织的HPV53也可致尖锐湿疣的发生。

Objective To identify and assess multiple human papillomavirus types in condyloma acuminatum lesions from patients with genital warts in Beijing area,and compare different features between otherwise healthy and immunosuppressed patients.Methods PCR,RFLP and nucleotide sequencing analysis were used to determine HPV types from individual lesions.Results The predominant type from other healthy patients was HPV6,secondly HPV11.The mean age of patients infected by HPV6was lower than that of HPV11and HPV6+11.While lesions from immunosuppressed patients were often contained HPV11or mixed with HPV6.Besides,HPV types16and53were detected from infected lesions than other HPV types.Conclusions HPV6was the major pathogen of condyloma acuminatum,but infected patients were at lower ages.While HPV11was most often detected from immunosuppressed patients.As a low risk virus in normal genital tract,HPV53also could be a pathogen in genital warts.