电针诱导大鼠脑缺血耐受作用的穴位特异性研究

Acupoint Specificity of Electroacupuncture Preconditioning Effect on Cerebral Ischemia Injury in Rats

目的 :探讨电针诱导大鼠脑缺血耐受作用的穴位特异性。方法 :4 0只雄性SD大鼠 ,随机分为 4组 ,每组各 10只 ,即空白组、戊巴比妥组、针刺肢体组和针刺“百会”组。最后一次处理 2 4h后采用颈内动脉尼龙线线栓法致右大脑中动脉栓塞 (12 0min)模型 ,观察再灌注后 2 4h时神经功能损害并取大脑行TTC染色以测量脑梗死容积。结果 :再灌注 2 4h时神经功能损害评分及脑梗死容积。针刺“百会”组均明显小于其余 3组 (P均 <0 0 5 ) ,针刺肢体组与两对照组无明显差异 (P >0 0 5 )。结论 :电针“百会”预处理减轻大鼠神经功能损害程度明显优于针刺肢体。

Purpose To investigate acupoint specificity of electroacupuncture preconditioning effect on cerebral ischemia. Methods 40 male SD rats were randomly divided into 4 groups, control group, pentobarbital group, needling limb group and needling point Baihui (GV 20) group, 10 rats each group. 24 hours after the last treatment, the right middle cerebral artery was occluded for 120 min, and the neurological outcome was evaluated after reperfusion for 24 hours, and then the cerebral infarct size was investigated with TTC staining. Results The neurological deficit score and the infarct size in the needling point Baihui group were lower than that in the other 3 groups, respectively, P<0.05; there was no significant difference as the needling limb group compared with the pentobarbital group or the control group, P>0.05. Conclusion Electroacupuncture at Baihui can reduce neurological injury induced by temporary middle cerebral artery occlusion and the effect is better than that of needling the limb.