HBeAg、HBV DNA斑点杂交以及定量PCR检测在评价干扰素抗HBV疗效的价值探讨

Effect of interferon on HBV determined by dot hybridization and PCR.

目的 免疫学检测 HBe Ag;斑点杂交法及 PCR定量检测法检测 HBV DNA评价干扰素抗 HBV的疗效。方法 对纳入治疗组的慢性乙肝患者治疗前后均同时检测乙肝两对半用两种方法检测血清 HBV DNA。结果 干扰素完全应答者 HBV DNA转阴较 HBe Ag发生早 ;HBV DNA定量在总体上呈下降趋势。 HBV DNA定量 PCR较斑点杂交更能反映干扰素治疗过程中患者外围血内病毒拷贝数的增长。结论 两种方法均能灵敏地反映干扰素抗 HBV的疗效 。

Objective To appraise the effect of interferon on HBV by dot hyhridization and quantitative PRC.Methods Two ways of HBV DNA detection were used to test the serum of patients wsho had received interferon treatment.Results HBV DNA turned neggative eartier than HBeAg did the quantave analysis of HBV DNA showed a decrease in all patients tyested With interferon,HBV guantitative PRC qrovided a more accurate determination of viral copies in the qrocess of interferon therapy than dot hybridization Conetusion HBV DNA detection is abte to determine the effect of interferon on HBV.It can be done by both quantitative PRC and dot hyhridization,either of which has its own advantages.