预处理对高原大鼠肝脏缺血再灌注损伤的防护作用

PRECONDITIONING AGAINST IS CHEMIA-REPERFUSION INJURY IN RAT LIVER

目的 :探讨丹参、地塞米松、L -精氨酸、维拉帕米在肝脏缺血预处理 (IPC)过程中的作用 ;方法 :在原位灌流的大鼠肝脏缺血再灌注模型上 ,经尾静脉注射丹参、地塞米松、L -精氨酸和维拉帕米 ,检测上述药物在IPC中的疗效 ;结果 :药物预处理组与对照组比较 ,药物预处理可阻止血清谷丙转氨酶 (ALT ,U/L) 1 3 7.7± 1 5.6、1 4 2 .6± 1 5.9、1 4 6.3±1 3 .7、1 4 2 .8± 1 5.7与 1 74.7± 2 3 .9;谷草转氨酶 ( (AST ,U/L) 2 63 .1± 2 3 .5、2 56.8± 2 8.7、2 72 .5± 2 2 .5、3 2 9.3± 3 6.5与 3 40 .2± 3 8.3 ;乳酸脱氢酶 (LDH ,U/L) 1 2 0 6± 1 3 7、1 2 96±1 1 8、1 2 2 0± 1 2 7、1 2 76± 1 2 3与 2 568± 1 53及脂质过氧化物 (MDA ,mmol/L) 2 9.7± 5.4、2 7.6± 4.5、2 7.8± 4.7、2 7.6± 5.7与 3 8.7± 7.6水平升高 (P <0 .0 1 ) ;而使超氧化歧化酶(SOD ,mu/L) 95.1± 1 0 .2、92 .4± 1 1 .4、92 .4± 1 0 .5、95.3± 1 0 .8与 73 .7± 5.4;一氧化氮(NO ,mmol/L) 81 .5± 7.9、86.4± 9.4、88.3± 9.6、76.3± 8.6与 64.2± 6.8保持在较高水平 (P <0 .0 1 ) ;药物预处理组肝脏病理改变程度明显轻于缺血再灌注组 ,但维拉帕米不如丹参、地塞米松、L -精氨酸的疗效 (P <0 .0 5) ;结论

To check the protection of ischemic preconditioning(IPC) and investigate the role of salvia miltiorrhiza and Dexamethasone L-arginine Verapamil in the process.Methods:On the model of rat liver subjected to ischemic reperfusion injury,we examined the medicine effect of Salvia miltiorrhiza dexamethasone L-arginine Verapamil by tail veil injected.Results: IPC prevented increase in ALT(137.7±15.6,142.6±15.9,146.3±13.7,142.8±15.7,174.7±23.9)U/L,AST(263.1±23.5,256.8±28.7,272.5±22.5,329.3±36.5)U/L,vs(340.2±38.3)U/L and LDH(1 206±137, 1 293 ±118,1 220±127,1 276±123)U/L,vs(2 568±153)U/L and in the level of MDA(29.7±5.4,27.6±4.5,27.8±4.7,27.6±5.7)mmol/L,vs(38.7±7.6)mmol/L in the tissuesve ischemia-reperfusion (P<0.01),and maintained the high level of SOD(95.1±10.2,92.4±11.4,92.4±10.5, 95.3 ± 10.8 )mu/L,vs(73.7±5.4)mu/L and Nitric oxide (81.5±7.9,86.4±9.4,88.3±9.6,76.3± 8.6 )mmol/L,vs(64.2±6.8)mmol/L in the tissuesve ischemia-reperfusion P<0.01).The pathologic change of preconditioming was lighter than the pathologic change of ischemic reperfusion.The effecttion of salvia miltiorrhiza dexamethasone L-arginine was better than verapamil′s P<0.05).Conclusions:IPC has protective effect on the liver function,the effects of salvia miltiorrhiza dexamethasone L-arginine was better than verapamil's.Preconditioning could reduce the release of the hepatic enzymes and MDA after liver ischemia-reperfusion injury,blood exchang and improve liver oxygen demand.