外周血单核细胞CD69/CD3比值在评估系统性红斑狼疮疾病活动性中的作用

Significance of CD69/CD3 ratio of peripheral blood mononuclear cells as a marker for evaluating the disease activity of systemic lupus erythematosus

目的 通过检测系统性红斑狼疮 (SLE)患者外周血中T细胞CD6 9表达 ,以探讨CD6 9/CD3比值在评估SLE疾病活动指数中的作用。方法 利用流式细胞分析法对 5 4例SLE患者和 18名健康志愿者外周血T细胞上CD6 9表达进行分析 ,同时检测患者抗dsDNA抗体、C3和C4水平。结果 SLE患者CD6 9/CD3的比值显著高于正常人群 (P <0 0 1) ,当抗dsDNA抗体、C3和C4水平与疾病活动性缺乏相关关系时 ,CD6 9/CD3仍与SLE活动指数 (SLEDAI)有明显相关性 (P <0 0 5 )。结论 T细胞活化 (CD6 9/CD3)程度与SLEDAI指数有明显的相关性 。

Objective To investigate whether the disease activity of systemic lupus erythematosus (SLE) is correlated with T cell activation.The CD69/CD3 ratio of peripheral blood mononuclear cells was measured.Methods The expression of CD69/CD3 on T cells from peripheral blood mononuclear cells was investigated in 54 SLE and 18 healthy volunteers by using flow cytometric analysis and immunofluorescence staining method.And the levels of anti dsDNA,complements C3 and C4 were also measured.Results Expression of CD69 on T cells from SLE patients and healthy controls showed significant difference ( P <0 01),and CD69/CD3 still showed high correlation with SLEDAI ( P <0 05) when levels of anti dsDNA,C3 and C4 failed to show good correlation.Conclusion CD69/CD3 which detects T cell activity is significantly correlated with SLEDAI scores and is a marker to monitor systemic lupus erythematosus disease activity.