门静脉转流对肝硬化大鼠耐受入肝血流阻断时限的影响

Effect of portal vein blood bypass on tolerance limit of hepatic inflow occlusion in rats with liver cirrhosis

目的 探讨门静脉转流对肝硬化大鼠耐受入肝血流阻断时限的影响。方法 观测门静脉转流与非门静脉转流条件下阻断入肝血流不同时程后动物存活率及肝脏能量代谢的变化 ,包括肝细胞线粒体呼吸控制率 (RCR)、肝组织三磷酸腺苷 (ATP)含量、动脉血酮体比值 (AKBR)。结果 ①术后两周存活率 :肝硬化大鼠门静脉转流下入肝血流阻断 (PBB) 3 0min组和PBB 45min组为 10 0 % ,单纯入肝血流阻断 (PTC) 3 0min组、PBB 60min组分别为 65 %和 75 %。②肝脏能量代谢 :缺血后肝组织ATP、RCR及AKBR均明显下降。再灌注后上述指标逐渐恢复 ,但随缺血时间延长 ,升高幅度明显降低 ,甚至难以恢复。

Objectives To study the effect of portal vein blood bypass (PBB) on the tolerance limit of rats with liver cirrhosis to hepatic inflow occlusion (HIO). Methods Animal survival rate and hepatic energy metabolism were observed following different period of HIO with the effect of PBB and without PBB, including respiratory controa ratio (RCR), adenosine tirphosphate (ATP) and arterial blood ketone body ratio (AKBR). Results ①Animal survival rate within 2 weeks: The rates of the animal with PBB were 100%, 100% and 75% respectively subjected to 30, 45 and 60 minutes' HIO. The group with 30 min of portal trial clamp (PTC) was 65%. ② Hepatic energy metabolism: ATP, RCR and AKBR were all descend obviously after ischemia. The parameters rose after reperfusion, but the degree of decrease was related to the prolongation of ischemia. Conclusion The tolerance limit of rats with liver cirrhosis to normothermic HIO is raised by PBB significantly.