缺氧及氧化损伤后GEC粘附分子表达与粘附性改变

Expressions of adhesion molecules on glomerular endothelial cells and changes of adhesion properties after anoxia/oxidative stress in vitro

目的 探讨缺氧、氧化损伤对培养人肾小球内皮细胞 (GEC)粘附分子表达及GEC与PMN粘附力的影响。方法 采用化学缺氧、氧化损伤模型 ,免疫荧光观察GEC粘附分子P、E选择素和ICAM 1的表达 ,微管吸吮直接测定单个GEC、PMN细胞间粘附力的改变。结果 缺氧、氧化损伤均可导致GEC细胞P、E选择素和ICAM 1的表达增强 ,以ICAM 1最明显 ,同时GEC、PMN间粘附力增强 ,加用ICAM 1抗体可显著抑制这一粘附力的增加。结论 缺氧、氧化损伤后GEC粘附分子表达增强 ,GEC。

Objective To explore the effects of anoxia/oxidative stress on the expressions of adhesion molecules on glomerular endothelial cells (GECs) and its adhesion properties. Methods Chemical anoxia/oxidative stress models of GECs were established, and the expressions of P selectin, E selectin and ICAM 1 in them were studied with immunofluorescence. Micropipette aspirate techniques were used to measure the adhesive forces between GECs and PMNs. Results Anoxia/oxidative stress increased the expression of P selectin, E selectin and ICAM 1, with the last one most obvious. Adhesive forces between GECs and PMNs increased simultaneously. Antibody to ICAM 1 inhibited the adhesive forces between GECs and PMNs markedly. Conclusion The expressions of adhesion molecules on GECs increased after anoxia/oxidative stress, and these changes are related to the changes of adhesive forces between GECs and PMNs.